Can moderation be tested even if the reduction in stress perception (tested in hypothesis 1) is not significant?

In my first hypothesis, I calculated a mixed anova with repeated measures to find out whether perceived stress (recorded at t1, t2, t3) is reduced after participation in a mindfulness intervention compared to a control group over the course of the three time points. The effect was not significant. Now I would like to test in my second hypothesis whether the reduction of the perceived stress score is moderated by the mindfulness score (recorded at t1, t2, t3) over the course of the three measurement points. Can I test the hypothesis even if I actually know that the reduction (tested in hypothesis 1) is not significant?
And if so, how can I proceed?

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