Error in running run_test_multiple_groups() function for microbiomeMarker package.

I'm trying to do "Anova" method by run_test_multiple_groups() in R package microbiomeMarker.
I ran the following code,

ps_anova <- phyloseq::subset_samples(ps,
    Hypertension_type_2 %in% c("Normal", "Prehypertensive", "Staged")
)
mg_anova <- run_test_multiple_groups(
  ps_anova,
  group = "Hypertension_type_2",
  method = "anova"
)
mg_anova

But the Error showing is
Screenshot from 2023-03-30 12-54-14

Here is the code I used to generate the phyloseq object ps

ps <- import_qiime2(
    otu_qza = "/media/turtle/New Volume/Nihad/Test_analysis_2/PRJNA417579-All/fondue-output/dada2_table.qza",
    taxa_qza = "/media/turtle/New Volume/Nihad/Test_analysis_2/PRJNA417579-All/fondue-output/taxonomy.qza",
    sam_tab = "/media/turtle/New Volume/Nihad/Test_analysis_2/PRJNA417579-All/fondue-output/PRJNA417579_final - final_metadata_EDITED.tsv",
    tree_qza = "/media/turtle/New Volume/Nihad/Test_analysis_2/PRJNA417579-All/fondue-output/rooted-tree.qza"
)
ps

And the files I've used to create this are in here.

Can anyone help me with this?

This topic was automatically closed 42 days after the last reply. New replies are no longer allowed.

If you have a query related to it or one of the replies, start a new topic and refer back with a link.