I've been trying to get a sigmoidal curve line that goes through/near my data points, so that i can use the sigmoidal fit to find the values of Km and Vmax. Im not sure how to do this. what i have of my code so far is:
s <- c (5, 10, 15, 50, 75, 100) v <- c (0.0372, 0.0431, 0.0788, 0.122, 0.131, 0.105) sigmoid <- nls( v ~ Vmax * s^h / ( Km + s^h ) , start = list ( Vmax =0.2, Km =50, h=1)) summary (sigmoid) # draw a basic sigmoidal plot plot ( v ∼ s, main = "" , ylab = " v (umol/min/mg protein) " , xlab = " [Ninhydrin Hydrate] (uM)" )
could i get some help please? thank you!