Hi,
I'm trying to put a gate on my scatter plot, but I'm running to the error: "Error in chol.default(cov) : the leading minor of order 2 is not positive definite"
I don't know what it means and I'm having trouble finding resources to understand it.
Here's my code!
meantBB<- c("BL1.H"=9, "BL3.H"=9)
cov <- matrix(c(7.5,7.9,9,10.5), ncol = 2, dimnames=list(c("BL1.H", "BL3.H"), c("BL1.H", "BL3.H")))
GateBB<- ellipsoidGate(.gate = cov,
mean = meantBB,
distance = 1,
filterId = "test gate")
ps_rose.0t <- ggcyto::ggcyto(InFCS_ta[rose.0], aes(x = `BL3.H`, y = `BL1.H`)) +
geom_hex(bins = 300) +
theme_bw()+
theme(axis.text = element_text(size = 12),
axis.title = element_text(size = 12),
strip.background = element_rect(colour="white", fill="white"),
panel.border = element_rect(colour = "white"))+
geom_gate(GateBB, col = "#CB0001", fill = "ffa401", alpha = 0.8, size = 1)
plot(ps_rose.0t, echo = FALSE)
Thanks!