Legend of geom_tile() isn't showing the last break value

General
1

So I'm trying to make a heatmap with geom_tile and I'm getting a problem with my legend. I put in breaks from 2 to -2 by 1, and the legend displays the values -1 through 2, but isn't displaying the -2 at the bottom. How can I fix this?


> dput(head(sample_plot))
list(data = structure(list(protein = c("Oxygen-evolving enhancer protein, chloroplastic", 
"Oxygen-evolving enhancer protein, chloroplastic", "Ribulose bisphosphate carboxylase small subunit", 
"Cluster of Oxygen-evolving enhancer protein, chloroplastic", 
"Oxygen-evolving enhancer protein, chloroplastic", "Oxygen-evolving enhancer protein, chloroplastic", 
"Uncharacterized Protein", "Cluster of Photosystem I reaction center subunit N, chloroplastic", 
"Photosystem I reaction center subunit, chloroplastic", "Phosphopentokinase", 
"Photosystem I reaction center subunit, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Photosystem II repair protein PSB27-H1, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Geranylgeranyl reductase", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem II reaction center Psb28 protein", 
"Photosystem II protein PSBR chloroplastic", "Mg-protoporphyrin IX chelatase", 
"Photosystem II core complex proteins psbY", "Magnesium chelatase", 
"Photosystem I reaction center subunit, chloroplastic", "Protein THYLAKOID FORMATION1, chloroplastic", 
"Uncharacterized Protein", "Chlorophyll a-b binding protein, chloroplastic", 
"PSI-F", "Chlorophyll a-b binding protein, chloroplastic", "Uncharacterized Protein", 
"NADPH-protochlorophyllide oxidoreductase", "Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem I reaction center subunit VI", 
"Ycf3-interacting protein, chloroplastic", "Mg-protoporphyrin IX chelatase", 
"Photosystem I reaction center subunit psaK, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Uncharacterized Protein", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem Ii D1 precursor processing protein, chloroplastic", 
"Uncharacterized Protein", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Photosynthetic NDH subunit of lumenal location, chloroplastic", 
"Heme oxygenase, chloroplastic", "Thylakoid lumenal 19 kDa protein, chloroplastic", 
"Magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase", 
"Uncharacterized Protein", "Photosystem I assembly factor, chloroplastic", 
"Uncharacterized Protein", "Protein PROTON GRADIENT REGULATION, chloroplastic", 
"PSI subunit V", "PSII 6.1 kDa protein", "Uncharacterized Protein", 
"Uncharacterized Protein", "Oxygen-evolving enhancer protein, chloroplastic", 
"Oxygen-evolving enhancer protein, chloroplastic", "Ribulose bisphosphate carboxylase small subunit", 
"Cluster of Oxygen-evolving enhancer protein, chloroplastic", 
"Oxygen-evolving enhancer protein, chloroplastic", "Oxygen-evolving enhancer protein, chloroplastic", 
"Uncharacterized Protein", "Cluster of Photosystem I reaction center subunit N, chloroplastic", 
"Photosystem I reaction center subunit, chloroplastic", "Phosphopentokinase", 
"Photosystem I reaction center subunit, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Photosystem II repair protein PSB27-H1, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Geranylgeranyl reductase", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem II reaction center Psb28 protein", 
"Photosystem II protein PSBR chloroplastic", "Mg-protoporphyrin IX chelatase", 
"Photosystem II core complex proteins psbY", "Magnesium chelatase", 
"Photosystem I reaction center subunit, chloroplastic", "Protein THYLAKOID FORMATION1, chloroplastic", 
"Uncharacterized Protein", "Chlorophyll a-b binding protein, chloroplastic", 
"PSI-F", "Chlorophyll a-b binding protein, chloroplastic", "Uncharacterized Protein", 
"NADPH-protochlorophyllide oxidoreductase", "Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem I reaction center subunit VI", 
"Ycf3-interacting protein, chloroplastic", "Mg-protoporphyrin IX chelatase", 
"Photosystem I reaction center subunit psaK, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Uncharacterized Protein", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem Ii D1 precursor processing protein, chloroplastic", 
"Uncharacterized Protein", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Photosynthetic NDH subunit of lumenal location, chloroplastic", 
"Heme oxygenase, chloroplastic", "Thylakoid lumenal 19 kDa protein, chloroplastic", 
"Magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase", 
"Uncharacterized Protein", "Photosystem I assembly factor, chloroplastic", 
"Uncharacterized Protein", "Protein PROTON GRADIENT REGULATION, chloroplastic", 
"PSI subunit V", "PSII 6.1 kDa protein", "Uncharacterized Protein", 
"Uncharacterized Protein", "Oxygen-evolving enhancer protein, chloroplastic", 
"Oxygen-evolving enhancer protein, chloroplastic", "Ribulose bisphosphate carboxylase small subunit", 
"Cluster of Oxygen-evolving enhancer protein, chloroplastic", 
"Oxygen-evolving enhancer protein, chloroplastic", "Oxygen-evolving enhancer protein, chloroplastic", 
"Uncharacterized Protein", "Cluster of Photosystem I reaction center subunit N, chloroplastic", 
"Photosystem I reaction center subunit, chloroplastic", "Phosphopentokinase", 
"Photosystem I reaction center subunit, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Photosystem II repair protein PSB27-H1, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Geranylgeranyl reductase", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem II reaction center Psb28 protein", 
"Photosystem II protein PSBR chloroplastic", "Mg-protoporphyrin IX chelatase", 
"Photosystem II core complex proteins psbY", "Magnesium chelatase", 
"Photosystem I reaction center subunit, chloroplastic", "Protein THYLAKOID FORMATION1, chloroplastic", 
"Uncharacterized Protein", "Chlorophyll a-b binding protein, chloroplastic", 
"PSI-F", "Chlorophyll a-b binding protein, chloroplastic", "Uncharacterized Protein", 
"NADPH-protochlorophyllide oxidoreductase", "Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem I reaction center subunit VI", 
"Ycf3-interacting protein, chloroplastic", "Mg-protoporphyrin IX chelatase", 
"Photosystem I reaction center subunit psaK, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Uncharacterized Protein", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem Ii D1 precursor processing protein, chloroplastic", 
"Uncharacterized Protein", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Photosynthetic NDH subunit of lumenal location, chloroplastic", 
"Heme oxygenase, chloroplastic", "Thylakoid lumenal 19 kDa protein, chloroplastic", 
"Magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase", 
"Uncharacterized Protein", "Photosystem I assembly factor, chloroplastic", 
"Uncharacterized Protein", "Protein PROTON GRADIENT REGULATION, chloroplastic", 
"PSI subunit V", "PSII 6.1 kDa protein", "Uncharacterized Protein", 
"Uncharacterized Protein", "Oxygen-evolving enhancer protein, chloroplastic", 
"Oxygen-evolving enhancer protein, chloroplastic", "Ribulose bisphosphate carboxylase small subunit", 
"Cluster of Oxygen-evolving enhancer protein, chloroplastic", 
"Oxygen-evolving enhancer protein, chloroplastic", "Oxygen-evolving enhancer protein, chloroplastic", 
"Uncharacterized Protein", "Cluster of Photosystem I reaction center subunit N, chloroplastic", 
"Photosystem I reaction center subunit, chloroplastic", "Phosphopentokinase", 
"Photosystem I reaction center subunit, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Photosystem II repair protein PSB27-H1, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Geranylgeranyl reductase", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem II reaction center Psb28 protein", 
"Photosystem II protein PSBR chloroplastic", "Mg-protoporphyrin IX chelatase", 
"Photosystem II core complex proteins psbY", "Magnesium chelatase", 
"Photosystem I reaction center subunit, chloroplastic", "Protein THYLAKOID FORMATION1, chloroplastic", 
"Uncharacterized Protein", "Chlorophyll a-b binding protein, chloroplastic", 
"PSI-F", "Chlorophyll a-b binding protein, chloroplastic", "Uncharacterized Protein", 
"NADPH-protochlorophyllide oxidoreductase", "Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem I reaction center subunit VI", 
"Ycf3-interacting protein, chloroplastic", "Mg-protoporphyrin IX chelatase", 
"Photosystem I reaction center subunit psaK, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Uncharacterized Protein", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem Ii D1 precursor processing protein, chloroplastic", 
"Uncharacterized Protein", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Photosynthetic NDH subunit of lumenal location, chloroplastic", 
"Heme oxygenase, chloroplastic", "Thylakoid lumenal 19 kDa protein, chloroplastic", 
"Magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase", 
"Uncharacterized Protein", "Photosystem I assembly factor, chloroplastic", 
"Uncharacterized Protein", "Protein PROTON GRADIENT REGULATION, chloroplastic", 
"PSI subunit V", "PSII 6.1 kDa protein", "Uncharacterized Protein", 
"Uncharacterized Protein", "Oxygen-evolving enhancer protein, chloroplastic", 
"Oxygen-evolving enhancer protein, chloroplastic", "Ribulose bisphosphate carboxylase small subunit", 
"Cluster of Oxygen-evolving enhancer protein, chloroplastic", 
"Oxygen-evolving enhancer protein, chloroplastic", "Oxygen-evolving enhancer protein, chloroplastic", 
"Uncharacterized Protein", "Cluster of Photosystem I reaction center subunit N, chloroplastic", 
"Photosystem I reaction center subunit, chloroplastic", "Phosphopentokinase", 
"Photosystem I reaction center subunit, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Photosystem II repair protein PSB27-H1, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Geranylgeranyl reductase", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem II reaction center Psb28 protein", 
"Photosystem II protein PSBR chloroplastic", "Mg-protoporphyrin IX chelatase", 
"Photosystem II core complex proteins psbY", "Magnesium chelatase", 
"Photosystem I reaction center subunit, chloroplastic", "Protein THYLAKOID FORMATION1, chloroplastic", 
"Uncharacterized Protein", "Chlorophyll a-b binding protein, chloroplastic", 
"PSI-F", "Chlorophyll a-b binding protein, chloroplastic", "Uncharacterized Protein", 
"NADPH-protochlorophyllide oxidoreductase", "Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem I reaction center subunit VI", 
"Ycf3-interacting protein, chloroplastic", "Mg-protoporphyrin IX chelatase", 
"Photosystem I reaction center subunit psaK, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Cluster of Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Uncharacterized Protein", 
"Chlorophyll a-b binding protein, chloroplastic", "Photosystem Ii D1 precursor processing protein, chloroplastic", 
"Uncharacterized Protein", "Chlorophyll a-b binding protein, chloroplastic", 
"Chlorophyll a-b binding protein, chloroplastic", "Chlorophyll a-b binding protein, chloroplastic", 
"Photosynthetic NDH subunit of lumenal location, chloroplastic", 
"Heme oxygenase, chloroplastic", "Thylakoid lumenal 19 kDa protein, chloroplastic", 
"Magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase", 
"Uncharacterized Protein", "Photosystem I assembly factor, chloroplastic", 
"Uncharacterized Protein", "Protein PROTON GRADIENT REGULATION, chloroplastic", 
"PSI subunit V", "PSII 6.1 kDa protein", "Uncharacterized Protein", 
"Uncharacterized Protein"), variable = structure(c(1L, 1L, 1L, 
1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 
1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 
1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 
1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 2L, 2L, 
2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 
2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 
2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 
2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 3L, 
3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 
3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 
3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 
3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 
4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 
4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 
4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 
4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 4L, 
4L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 
5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 
5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 
5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 5L, 
5L, 5L), levels = c("Initial Biomass", "Anaerobic_24 hr", "Anaerobic_28 day", 
"Citric_24 hr", "Citric_28 day"), class = "factor"), value = c(0.0229, 
-0.012, -0.0014, -0.0192, -0.0152, -0.01645, 0.01955, 0.0022, 
0.0022, -0.0024, 0.00765, -0.00615, 0.00295, -0.00175, -5.00008e-05, 
0.0015, 0.0067, 0.00065, -0.003, -0.000800001, 0.000100002, -0.00335, 
0.0136, 0.01315, 0.0045, -0.01395, 0.0049, 0.00635, 0.0208, 0.00625, 
-0.00435, -0.00435, -0.02265, -0.00125, 0.000949999, -0.0161, 
-0.0161, -0.01475, 0.0052, 0.0407, 0.0029, 0.00835, 0.00395, 
0.0081, 0.016, 0.016, 0.00985, 0.002, 0.00605, 0, 0.00685, 0.0018, 
-0.00115, 0.00575, -0.000149999, 0.0071, 0.001, 0.0038, -0.0068, 
0.0123, 0.00045, 0.0045, 0.0011, -0.0017, 0.00035, -0.7887, 0.20305, 
0.05815, -0.97975, -0.88765, -1.09325, 0.26735, -1.19135, -1.19135, 
0.6408, 0.1516, 0.48605, 0.10135, 0.40935, -0.6456, 0.1359, -0.2903, 
0.1443, 0.24505, 0.19735, -0.61405, -0.0443, -0.08015, -0.21535, 
0.1462, 0.08925, -0.1323, -0.1227, 0.98755, -0.15245, 0.5417, 
0.5001, 0.6405, 0.19795, -0.00075, 0.2408, 0.2408, 0.42385, 0.3117, 
-0.26845, 0.5019, 0.2082, -1.67225, -0.0449, -0.1679, -0.1679, 
-0.38885, -0.52665, 0.37055, 0.2626, 0.1487, 0.29275, 0.22645, 
0.05735, 0.319, -0.0855, 0.0344, 0.40415, -0.06605, 0.01535, 
0.20435, -0.16365, 0.3687, -0.1238, 0.34685, -0.81635, -0.95825, 
0.76245, -1.5131, -1.5354, -1.46595, -1.2462, -1.9682, -1.9682, 
0.5832, -1.81145, 1.0311, -1.24, 1.25135, -0.32925, 1.57405, 
0.39985, -1.12845, -0.71595, 1.34155, -1.7564, 1.0713, 0.32505, 
0.77295, -1.01995, 0.772, 0.7041, 0.62095, -0.65415, 1.04235, 
0.59235, 0.76915, -0.03085, 0.5989, -0.07855, 1.0571, 1.0571, 
0.69125, 0.81565, -0.51465, -0.19885, 1.555, -1.0545, 0.47155, 
0.68315, 0.68315, -0.0827, -0.4303, -1.1624, 0.3803, 0.65735, 
0.6649, 0.7997, -0.285, 0.2409, -0.93155, 0.89655, 0.27775, 0.79715, 
0.2185, 0.02425, 0.86485, 0.91405, 0.0776, -1.8399, 0.04795, 
0.33515, 0.17345, -0.58095, -0.56835, -0.60585, -0.06985, 0.4739, 
0.4739, 0.23695, 0.16525, 0.19655, 0.0534, 0.30035, 0.0322, 0.0306, 
0.1894, 0.111, 0.35255, 0.0288, -0.2983, 0.0583, 0.08685, 0.35035, 
0.2282, 0.44345, -0.0763, 0.11595, 2.09285, -0.156, 0.40845, 
0.42925, 0.35805, 0.14805, 0.29525, 0.14835, 0.14835, 0.23895, 
0.24125, 0.2985, 0.23115, -0.1728, 0.82395, 0.1735, 0.2599, 0.2599, 
0.23955, -0.1563, 0.38225, 0.3673, 0.3696, 0.33545, 0.28135, 
0.1546, 0.1255, 0.3758, 0.23515, 0.1696, -0.09575, 0.1967, 0.22675, 
0.08775, 0.0979, -0.0855, 0.357, -0.36465, 0.01785, -0.000149999, 
-0.9437, -0.87165, -1.05605, -1.3784, -0.83705, -0.83705, 1.0222, 
-1.45525, 0.41715, -0.55185, 0.38445, -0.7846, 0.8979, -0.05835, 
-0.3824, -1.12885, 0.36885, -1.6932, 0.50965, 0.15745, 0.20675, 
-0.73525, 0.021, 0.0473, 0.0952, -1.12165, 0.5354, 1.01705, 1.32905, 
-0.0823, -0.19345, -0.0625, 0.33195, 0.33195, -0.05375, 0.2794, 
-1.06845, 0.0657, 0.3072, -1.6379, -0.15495, 0.14565, 0.14565, 
0.22885, -0.8642, -0.8482, 0.3639, 0.0399, 0.1023, -0.13285, 
0.0269, 0.0828, -0.3297, 0.2718, 0.1255, 0.0478, 0.24405, -0.35665, 
-0.08645, -0.14, 0.1836, -0.46445)), row.names = c(NA, -325L), class = "data.frame"), 
    layers = list(<environment>), scales = <environment>, mapping = structure(list(
        x = ~variable, y = ~protein, fill = ~value), class = "uneval"), 
    theme = list(), coordinates = <environment>)

The image displays as below:

Rplot
Rplot1057×573 33.3 KB

2

In your data, it looks like fill values range from -1.96 to 2.09. Therefore, in your plot legend, the color extends a little beyond 2 and doesn't quite drop to -2. Try adding the following line to your ggplot.

scale_fill_continuous(limits = c(-2, 2))
1 Like
3

That worked, thanks!

1 Like
4

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