Thanks for your reply. actually I am trying to construct the genetic map with a set of SNP markers and I use the ASMap package and adapting their package to my data the data loading was fine and my script from beginning is as below
library(ASMap)
> poptype='RIL5'
> ASMpvalue=1e-6
> mstMC <- mstmap.data.frame(data, pop.type = poptype, dist.fun = "kosambi",objective.fun = "COUNT", p.value = ASMpvalue, noMap.dist = 30, noMap.size = 2,miss.thresh = 10, mvest.bc = FALSE, traces=TRUE,as.cross=TRUE)
Error in mstmap.data.frame(data, pop.type = poptype, dist.fun = "kosambi", :
Non-allowable allele encodings in RIL marker set
The few columns of data is as below
| Marker |
101A |
101B |
101C |
101D |
101E |
101F |
101G |
101H |
102A |
102B |
102C |
102D |
102E |
102F |
102G |
102H |
103A |
103B |
103C |
103D |
103E |
103F |
103G |
103H |
104A |
104B |
104C |
104D |
104E |
104F |
104G |
104H |
105A |
105C |
105D |
105E |
105F |
105G |
105H |
106A |
106B |
106C |
106D |
106E |
106F |
106G |
106H |
107A |
107B |
107C |
107D |
107E |
107F |
107H |
108A |
108B |
108C |
108E |
108F |
108G |
108H |
109A |
109B |
109C |
109D |
109E |
109F |
109G |
109H |
110A |
110B |
110C |
110D |
110E |
110F |
110G |
110H |
111A |
111B |
111D |
111E |
111F |
111G |
112A |
112B |
112D |
112E |
112F |
112G |
112H |
113A |
113B |
113C |
113D |
113E |
113H |
114A |
114B |
114C |
114E |
114F |
114G |
114H |
115A |
115B |
115C |
115D |
115E |
115F |
115G |
115H |
116A |
116B |
116C |
116D |
116F |
116G |
116H |
117A |
117B |
117C |
117D |
117E |
117F |
117G |
117H |
118A |
118B |
118C |
118D |
118F |
118G |
118H |
119A |
119B |
119C |
119D |
119E |
119F |
119G |
119H |
120A |
120C |
120D |
120E |
120F |
120G |
120H |
121B |
121C |
121D |
121E |
121F |
121G |
122B |
122C |
122D |
122E |
122F |
122G |
122H |
123A |
123B |
123C |
123D |
123E |
123G |
123H |
124A |
124B |
124C |
124D |
124E |
124F |
124G |
124H |
| BS00023201_51 |
A |
A |
B |
A |
B |
B |
B |
B |
A |
A |
B |
B |
B |
B |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
B |
A |
A |
A |
B |
B |
A |
na |
B |
A |
A |
B |
A |
A |
B |
B |
A |
B |
A |
A |
B |
A |
A |
A |
na |
A |
A |
A |
B |
B |
B |
A |
A |
A |
A |
B |
A |
A |
A |
A |
A |
A |
A |
A |
A |
B |
A |
B |
B |
na |
B |
A |
B |
A |
B |
B |
B |
A |
B |
A |
A |
B |
A |
B |
B |
B |
A |
B |
B |
B |
A |
A |
B |
B |
A |
B |
A |
B |
B |
A |
A |
B |
A |
A |
B |
A |
B |
A |
B |
B |
B |
B |
A |
A |
B |
A |
A |
A |
B |
B |
A |
B |
A |
A |
A |
B |
A |
B |
A |
A |
B |
B |
A |
A |
B |
B |
A |
A |
B |
B |
B |
A |
A |
A |
A |
B |
B |
A |
A |
A |
A |
B |
B |
B |
B |
A |
B |
B |
A |
B |
A |
A |
B |
B |
A |
B |
A |
B |
A |
A |
B |
| AX-110068701 |
A |
A |
B |
A |
B |
B |
B |
B |
A |
A |
B |
B |
B |
B |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
B |
A |
A |
A |
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B |
A |
A |
B |
A |
A |
B |
A |
A |
B |
B |
A |
B |
A |
B |
B |
A |
A |
A |
na |
A |
A |
A |
B |
B |
B |
A |
A |
A |
A |
B |
A |
A |
A |
A |
A |
A |
A |
A |
A |
B |
A |
B |
B |
na |
B |
A |
B |
A |
B |
B |
B |
A |
B |
A |
A |
B |
A |
B |
B |
B |
A |
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A |
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A |
B |
B |
A |
B |
A |
A |
B |
B |
A |
B |
A |
B |
A |
A |
B |
| wsnp_Ex_rep_c106111_90308719 |
A |
A |
B |
A |
B |
B |
B |
B |
A |
A |
B |
B |
B |
B |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
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A |
A |
A |
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B |
A |
A |
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A |
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A |
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A |
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A |
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B |
A |
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A |
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B |
A |
B |
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A |
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B |
A |
A |
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A |
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A |
A |
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A |
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A |
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A |
A |
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B |
B |
A |
A |
A |
A |
B |
B |
A |
A |
A |
A |
B |
B |
B |
B |
A |
B |
B |
A |
B |
A |
A |
B |
B |
A |
B |
A |
B |
A |
A |
B |
| RFL_Contig1027_442 |
A |
A |
B |
A |
B |
B |
B |
B |
A |
A |
B |
B |
B |
B |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
B |
A |
A |
A |
B |
B |
A |
na |
B |
A |
A |
B |
A |
A |
B |
B |
A |
B |
A |
B |
B |
A |
A |
A |
na |
A |
A |
A |
B |
B |
B |
A |
A |
A |
A |
B |
A |
A |
A |
A |
A |
A |
A |
A |
A |
B |
A |
B |
B |
na |
B |
A |
B |
A |
B |
B |
B |
A |
B |
A |
A |
B |
A |
B |
B |
B |
A |
B |
B |
B |
A |
A |
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A |
A |
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A |
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A |
A |
A |
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A |
A |
A |
A |
B |
B |
B |
B |
A |
B |
B |
A |
B |
A |
A |
B |
B |
A |
B |
A |
B |
A |
A |
B |
| Excalibur_c5139_198 |
A |
A |
B |
A |
B |
B |
B |
B |
A |
A |
B |
B |
B |
B |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
B |
A |
A |
A |
B |
B |
A |
A |
B |
A |
A |
B |
A |
A |
B |
B |
A |
B |
A |
A |
B |
A |
A |
A |
na |
A |
A |
A |
B |
B |
B |
A |
A |
A |
A |
B |
A |
A |
A |
A |
A |
A |
A |
A |
A |
B |
A |
B |
B |
na |
B |
A |
B |
A |
B |
B |
B |
A |
B |
A |
A |
B |
A |
B |
B |
B |
A |
B |
B |
B |
A |
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| BobWhite_c39996_85 |
A |
A |
B |
A |
B |
B |
B |
B |
A |
A |
B |
B |
B |
B |
A |
B |
B |
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A |
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A |
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A |
na |
B |
A |
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A |
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A |
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na |
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A |
A |
A |
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A |
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na |
A |
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A |
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B |
na |
B |
A |
A |
B |
B |
A |
B |
A |
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A |
A |
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| Tdurum_contig43943_1165 |
A |
A |
B |
A |
B |
B |
B |
B |
A |
A |
B |
B |
B |
A |
A |
na |
B |
B |
A |
A |
B |
A |
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A |
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A |
A |
A |
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A |
na |
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A |
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| Tdurum_contig43943_56 |
A |
A |
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A |
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A |
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A |
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na |
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| AX-158560691 |
A |
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A |
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| AX-94844830 |
A |
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na |
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A |
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A |
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A |
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B |
A |
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B |
A |
B |
B |
A |
A |
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| TA005766-0499 |
A |
A |
A |
A |
B |
A |
A |
A |
B |
A |
A |
B |
A |
B |
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A |
A |
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A |
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A |
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A |
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A |
B |
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B |
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A |
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A |
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na |
B |
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A |
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A |
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A |
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A |
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A |
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| AX-158536828 |
A |
A |
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A |
B |
B |
B |
B |
A |
A |
B |
B |
B |
A |
A |
B |
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na |
A |
A |
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A |
B |
A |
B |
B |
A |
A |
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A |
na |
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A |
B |
B |
A |
B |
A |
A |
B |
B |
A |
A |
A |
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A |
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| AX-110368802 |
A |
A |
B |
A |
A |
B |
B |
B |
A |
A |
B |
B |
B |
A |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
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B |
A |
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A |
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B |
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A |
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B |
B |
B |
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A |
B |
B |
A |
B |
A |
A |
B |
B |
A |
A |
A |
B |
A |
A |
B |
| Tdurum_contig42479_3800 |
A |
A |
B |
A |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
na |
B |
na |
A |
B |
B |
A |
B |
A |
B |
na |
A |
A |
A |
B |
B |
A |
A |
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A |
A |
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A |
A |
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A |
na |
A |
B |
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B |
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B |
B |
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A |
A |
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A |
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na |
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A |
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A |
B |
B |
A |
B |
A |
A |
B |
B |
A |
A |
A |
A |
A |
A |
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| AX-158537535 |
A |
A |
B |
A |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
B |
B |
na |
A |
B |
B |
A |
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A |
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A |
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B |
B |
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A |
B |
B |
A |
B |
A |
A |
B |
B |
A |
A |
A |
A |
A |
A |
B |
| Ku_c4823_673 |
A |
A |
B |
A |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
B |
B |
B |
A |
B |
B |
A |
B |
A |
B |
B |
A |
A |
A |
B |
B |
A |
na |
B |
A |
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A |
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A |
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A |
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B |
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na |
B |
A |
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A |
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B |
B |
B |
A |
B |
B |
A |
B |
A |
A |
B |
B |
A |
A |
A |
A |
A |
A |
B |
| wsnp_Ex_c1137_2182795 |
A |
A |
B |
A |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
B |
B |
B |
A |
B |
B |
A |
B |
A |
B |
B |
A |
A |
A |
B |
B |
A |
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A |
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A |
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B |
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A |
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A |
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B |
A |
B |
B |
na |
B |
A |
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A |
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A |
B |
B |
A |
B |
A |
A |
B |
B |
A |
A |
A |
A |
A |
A |
B |
| AX-158544779 |
A |
A |
B |
A |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
B |
B |
B |
A |
B |
B |
A |
B |
A |
B |
B |
A |
A |
A |
B |
B |
A |
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A |
A |
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B |
B |
A |
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A |
A |
A |
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A |
A |
A |
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A |
A |
B |
B |
A |
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A |
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B |
A |
A |
A |
A |
A |
A |
A |
B |
A |
B |
A |
B |
B |
na |
B |
A |
A |
A |
B |
B |
B |
A |
A |
A |
A |
A |
B |
A |
B |
A |
A |
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B |
A |
A |
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A |
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A |
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B |
B |
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B |
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A |
A |
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A |
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B |
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A |
A |
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A |
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A |
A |
B |
B |
B |
B |
A |
B |
B |
A |
A |
A |
A |
B |
B |
A |
A |
A |
A |
A |
A |
B |
| Tdurum_contig83113_134 |
A |
A |
B |
A |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
B |
B |
B |
A |
B |
B |
A |
B |
A |
B |
B |
A |
A |
A |
B |
B |
A |
A |
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A |
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A |
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A |
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A |
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A |
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A |
A |
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B |
A |
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A |
A |
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A |
A |
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B |
A |
B |
A |
B |
B |
na |
B |
A |
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A |
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A |
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B |
B |
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A |
B |
B |
A |
A |
A |
A |
B |
B |
B |
A |
A |
A |
A |
A |
B |
| Tdurum_contig50845_82 |
A |
A |
B |
A |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
B |
B |
B |
A |
B |
B |
A |
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B |
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A |
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A |
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A |
B |
na |
B |
A |
B |
B |
na |
B |
A |
A |
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A |
A |
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A |
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A |
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B |
A |
A |
A |
A |
B |
B |
B |
A |
A |
A |
A |
A |
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| AX-94742021 |
A |
A |
B |
A |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
B |
B |
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A |
B |
B |
A |
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A |
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A |
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A |
B |
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A |
A |
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B |
B |
B |
A |
A |
A |
A |
A |
B |
| Tdurum_contig61492_684 |
na |
A |
B |
A |
A |
B |
B |
B |
A |
A |
B |
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A |
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A |
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B |
A |
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A |
A |
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A |
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A |
A |
A |
B |
A |
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A |
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A |
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| AX-110956659 |
A |
A |
B |
A |
A |
B |
B |
B |
A |
A |
B |
A |
B |
A |
A |
B |
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| AX-94952968 |
A |
A |
B |
A |
A |
B |
B |
B |
A |
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B |
A |
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A |
A |
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B |
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A |
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A |
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A |
A |
A |
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| BS00003761_51 |
na |
A |
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B |
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If you need any more clarifications please let me know
Thanks