A simple example.
library(ggplot2)
library(dplyr)
DF <- data.frame(Grp=c("A","A","A","A","B","B","B","B"),
Repl=c(1,1,2,2,1,1,2,2),
xValue=c(1,2,1,2,1,2,1,2),
yValue=c(1,2,1.5,2.2,1.25,2.3,0.5,3))
DF
#> Grp Repl xValue yValue
#> 1 A 1 1 1.00
#> 2 A 1 2 2.00
#> 3 A 2 1 1.50
#> 4 A 2 2 2.20
#> 5 B 1 1 1.25
#> 6 B 1 2 2.30
#> 7 B 2 1 0.50
#> 8 B 2 2 3.00
DF <- DF |> mutate(GrpRepl=paste(Grp,Repl,sep = "_"))
ggplot(DF,aes(xValue,yValue,color=Grp,group=GrpRepl))+geom_line()

Created on 2022-06-21 by the reprex package (v2.0.1)