Maybe it is because you forgot to save the results?
bubble_data = tibble(
Time = c("01.01.2022 12:34",
"02.01.2022 11:15",
"03.01.2022 11:53"),
Data1 = c(1,2,3),
Data2 = c(494959, 228984, 258588)
)
bubble_data2 = bubble_data %>%
separate(Time, into = c('Date', 'Time'), sep = " ")
bubble_data2
# A tibble: 3 x 4
Date Time Data1 Data2
<chr> <chr> <dbl> <dbl>
1 01.01.2022 12:34 1 494959
2 02.01.2022 11:15 2 228984
3 03.01.2022 11:53 3 258588