Here are some ideas for graphing using the ggplot2 library. I you have just one measurement for each combination of Location and Sublocation
library(ggplot2)
ggplot(DF, aes(x = Location, y = Flux, fill = Sublocation)) +
geom_col(position = "dodge")
If you have several reading at each combination
ggplot(DF, aes(x = Location, y = Flux, fill = Sublocation)) +
geom_boxplot()